Rv3795 - membrane indolylacetylinositol arabinosyltransferase embB


Protein Domains

Gene Information
LocusRv3795
SymbolembB
Gene Namemembrane indolylacetylinositol arabinosyltransferase embB
Location4246514 - 4249810 (+)
SpeciesMycobacterium tuberculosis H37Rv complete genome.
LengthGene:3297 bp
Protein:1099 aa
External LinksTuberculist
Target Gene Information
String Protein-Protein Interactions
STITCH Chemical-Protein Interactions
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Orthologs
Orthogroup Number14071
Related GenesMkms_5091 Mkms_5092 Mkms_5093 Mmcs_5003 Mmcs_5004 Mmcs_5005 MSMEG_6387 MSMEG_6389 MT3902 MUL_4974 Mvan_5630 Mvan_5632
Transcriptional Regulation
Operons View gene in operon browser
Regulatory Network
Search for regulators of Rv3795
Expression Correlation Genes with Correlated Expression
Scatterplot of Gene Expression

Sequence
Proteins
Genomic Sequence
Community Annotations Pending Curatorial Review
FieldValueStatusCreatorDate
TermTBRXN:AFE arabinofuranosyl extension (M tb) - IPIactivenjamshidi2012-10-05
This is a lumped reaction involving the emb locus (PMID: 9142129), details of the reaction are not well known. Recently it has been shown that the AftA locus 'primes' the araf extension (see AFTA) (PMID: 16595677), other sources imply that extension of ARaf residues ccurs while bound to the polyprenyl core (PMID: 11555614, Cole et al text Tuberculosis and the tubercle bacillus). Also note that this is the target of ethambutol (hence the locus name).
authors,DC. Crick,S. Mahapatra,PJ. Brennan Biosynthesis of the arabinogalactan-peptidoglycan complex of Mycobacterium tuberculosis. Glycobiology 2001
TermTBRXN:AFE arabinofuranosyl extension (M tb) - IPIactivenjamshidi2012-10-05
This is a lumped reaction involving the emb locus (PMID: 9142129), details of the reaction are not well known. Recently it has been shown that the AftA locus 'primes' the araf extension (see AFTA) (PMID: 16595677), other sources imply that extension of ARaf residues ccurs while bound to the polyprenyl core (PMID: 11555614, Cole et al text Tuberculosis and the tubercle bacillus). Also note that this is the target of ethambutol (hence the locus name).
LJ. Alderwick, M. Seidel et al. Identification of a novel arabinofuranosyltransferase (AftA) involved in cell wall arabinan biosynthesis in Mycobacterium tuberculosis. J. Biol. Chem. 2006
TermTBRXN:AFE arabinofuranosyl extension (M tb) - IPIactivenjamshidi2012-10-05
This is a lumped reaction involving the emb locus (PMID: 9142129), details of the reaction are not well known. Recently it has been shown that the AftA locus 'primes' the araf extension (see AFTA) (PMID: 16595677), other sources imply that extension of ARaf residues ccurs while bound to the polyprenyl core (PMID: 11555614, Cole et al text Tuberculosis and the tubercle bacillus). Also note that this is the target of ethambutol (hence the locus name).
A. Telenti, WJ. Philipp et al. The emb operon, a gene cluster of Mycobacterium tuberculosis involved in resistance to ethambutol. Nat. Med. 1997
TermTBRXN:AFE arabinofuranosyl extension (M tb) - ISSactivenjamshidi2012-10-05
This is a lumped reaction involving the emb locus (PMID: 9142129), details of the reaction are not well known. Recently it has been shown that the AftA locus 'primes' the araf extension (see AFTA) (PMID: 16595677), other sources imply that extension of ARaf residues ccurs while bound to the polyprenyl core (PMID: 11555614, Cole et al text Tuberculosis and the tubercle bacillus). Also note that this is the target of ethambutol (hence the locus name).
authors,DC. Crick,S. Mahapatra,PJ. Brennan Biosynthesis of the arabinogalactan-peptidoglycan complex of Mycobacterium tuberculosis. Glycobiology 2001
TermTBRXN:AFE arabinofuranosyl extension (M tb) - ISSactivenjamshidi2012-10-05
This is a lumped reaction involving the emb locus (PMID: 9142129), details of the reaction are not well known. Recently it has been shown that the AftA locus 'primes' the araf extension (see AFTA) (PMID: 16595677), other sources imply that extension of ARaf residues ccurs while bound to the polyprenyl core (PMID: 11555614, Cole et al text Tuberculosis and the tubercle bacillus). Also note that this is the target of ethambutol (hence the locus name).
LJ. Alderwick, M. Seidel et al. Identification of a novel arabinofuranosyltransferase (AftA) involved in cell wall arabinan biosynthesis in Mycobacterium tuberculosis. J. Biol. Chem. 2006
TermTBRXN:AFE arabinofuranosyl extension (M tb) - ISSactivenjamshidi2012-10-05
This is a lumped reaction involving the emb locus (PMID: 9142129), details of the reaction are not well known. Recently it has been shown that the AftA locus 'primes' the araf extension (see AFTA) (PMID: 16595677), other sources imply that extension of ARaf residues ccurs while bound to the polyprenyl core (PMID: 11555614, Cole et al text Tuberculosis and the tubercle bacillus). Also note that this is the target of ethambutol (hence the locus name).
A. Telenti, WJ. Philipp et al. The emb operon, a gene cluster of Mycobacterium tuberculosis involved in resistance to ethambutol. Nat. Med. 1997
InteractionRegulatory Rv1267cactiveashwinigbhat2012-10-05
Co-expression (Functional linkage)
AG. Amin, R. Goude et al. EmbA is an essential arabinosyltransferase in Mycobacterium tuberculosis. Microbiology (Reading, Engl.) 2008
InteractionRegulatory Rv1267cactiveashwinigbhat2012-10-05
Co-expression (Functional linkage)
authors,LJ. Alderwick,HL. Birch,AK. Mishra,L. Eggeling,GS. Besra Structure, function and biosynthesis of the Mycobacterium tuberculosis cell wall: arabinogalactan and lipoarabinomannan assembly with a view to discovering new drug targets. Biochem. Soc. Trans. 2007
InteractionRegulatory Rv1267cactiveashwinigbhat2012-10-05
Co-expression (Functional linkage)
VE. Escuyer, MA. Lety et al. The role of the embA and embB gene products in the biosynthesis of the terminal hexaarabinofuranosyl motif of Mycobacterium smegmatis arabinogalactan. J. Biol. Chem. 2001
InteractionRegulatory Rv1267cactivepriti.priety2012-10-05
Co-expression (Functional linkage)
AG. Amin, R. Goude et al. EmbA is an essential arabinosyltransferase in Mycobacterium tuberculosis. Microbiology (Reading, Engl.) 2008
InteractionRegulatory Rv1267cactivepriti.priety2012-10-05
Co-expression (Functional linkage)
authors,LJ. Alderwick,HL. Birch,AK. Mishra,L. Eggeling,GS. Besra Structure, function and biosynthesis of the Mycobacterium tuberculosis cell wall: arabinogalactan and lipoarabinomannan assembly with a view to discovering new drug targets. Biochem. Soc. Trans. 2007
InteractionRegulatory Rv1267cactivepriti.priety2012-10-05
Co-expression (Functional linkage)
VE. Escuyer, MA. Lety et al. The role of the embA and embB gene products in the biosynthesis of the terminal hexaarabinofuranosyl motif of Mycobacterium smegmatis arabinogalactan. J. Biol. Chem. 2001
InteractionPhysicalInteraction Rv3794activeashwinigbhat2012-10-05
Structural Analysis
AG. Amin, R. Goude et al. EmbA is an essential arabinosyltransferase in Mycobacterium tuberculosis. Microbiology (Reading, Engl.) 2008
InteractionPhysicalInteraction Rv3794activeashwinigbhat2012-10-05
Structural Analysis
authors,LJ. Alderwick,HL. Birch,AK. Mishra,L. Eggeling,GS. Besra Structure, function and biosynthesis of the Mycobacterium tuberculosis cell wall: arabinogalactan and lipoarabinomannan assembly with a view to discovering new drug targets. Biochem. Soc. Trans. 2007
InteractionPhysicalInteraction Rv3794activeashwinigbhat2012-10-05
Structural Analysis
VE. Escuyer, MA. Lety et al. The role of the embA and embB gene products in the biosynthesis of the terminal hexaarabinofuranosyl motif of Mycobacterium smegmatis arabinogalactan. J. Biol. Chem. 2001
InteractionPhysicalInteraction Rv3794activeashwinigbhat2012-10-05
Spectrophotometric
AG. Amin, R. Goude et al. EmbA is an essential arabinosyltransferase in Mycobacterium tuberculosis. Microbiology (Reading, Engl.) 2008
InteractionPhysicalInteraction Rv3794activeashwinigbhat2012-10-05
Spectrophotometric
authors,LJ. Alderwick,HL. Birch,AK. Mishra,L. Eggeling,GS. Besra Structure, function and biosynthesis of the Mycobacterium tuberculosis cell wall: arabinogalactan and lipoarabinomannan assembly with a view to discovering new drug targets. Biochem. Soc. Trans. 2007
InteractionPhysicalInteraction Rv3794activeashwinigbhat2012-10-05
Spectrophotometric
VE. Escuyer, MA. Lety et al. The role of the embA and embB gene products in the biosynthesis of the terminal hexaarabinofuranosyl motif of Mycobacterium smegmatis arabinogalactan. J. Biol. Chem. 2001
InteractionPhysicalInteraction Rv3794activepriti.priety2012-10-05
Structural Analysis
AG. Amin, R. Goude et al. EmbA is an essential arabinosyltransferase in Mycobacterium tuberculosis. Microbiology (Reading, Engl.) 2008
InteractionPhysicalInteraction Rv3794activepriti.priety2012-10-05
Structural Analysis
authors,LJ. Alderwick,HL. Birch,AK. Mishra,L. Eggeling,GS. Besra Structure, function and biosynthesis of the Mycobacterium tuberculosis cell wall: arabinogalactan and lipoarabinomannan assembly with a view to discovering new drug targets. Biochem. Soc. Trans. 2007
InteractionPhysicalInteraction Rv3794activepriti.priety2012-10-05
Structural Analysis
VE. Escuyer, MA. Lety et al. The role of the embA and embB gene products in the biosynthesis of the terminal hexaarabinofuranosyl motif of Mycobacterium smegmatis arabinogalactan. J. Biol. Chem. 2001
InteractionPhysicalInteraction Rv3794activepriti.priety2012-10-05
Spectrophotometric
AG. Amin, R. Goude et al. EmbA is an essential arabinosyltransferase in Mycobacterium tuberculosis. Microbiology (Reading, Engl.) 2008
InteractionPhysicalInteraction Rv3794activepriti.priety2012-10-05
Spectrophotometric
authors,LJ. Alderwick,HL. Birch,AK. Mishra,L. Eggeling,GS. Besra Structure, function and biosynthesis of the Mycobacterium tuberculosis cell wall: arabinogalactan and lipoarabinomannan assembly with a view to discovering new drug targets. Biochem. Soc. Trans. 2007
InteractionPhysicalInteraction Rv3794activepriti.priety2012-10-05
Spectrophotometric
VE. Escuyer, MA. Lety et al. The role of the embA and embB gene products in the biosynthesis of the terminal hexaarabinofuranosyl motif of Mycobacterium smegmatis arabinogalactan. J. Biol. Chem. 2001
InteractionPhysicalInteraction Rv3794activeashwinigbhat2012-10-05

AG. Amin, R. Goude et al. EmbA is an essential arabinosyltransferase in Mycobacterium tuberculosis. Microbiology (Reading, Engl.) 2008
InteractionPhysicalInteraction Rv3794activeashwinigbhat2012-10-05

authors,LJ. Alderwick,HL. Birch,AK. Mishra,L. Eggeling,GS. Besra Structure, function and biosynthesis of the Mycobacterium tuberculosis cell wall: arabinogalactan and lipoarabinomannan assembly with a view to discovering new drug targets. Biochem. Soc. Trans. 2007
InteractionPhysicalInteraction Rv3794activeashwinigbhat2012-10-05

VE. Escuyer, MA. Lety et al. The role of the embA and embB gene products in the biosynthesis of the terminal hexaarabinofuranosyl motif of Mycobacterium smegmatis arabinogalactan. J. Biol. Chem. 2001
InteractionPhysicalInteraction Rv3794activepriti.priety2012-10-05

AG. Amin, R. Goude et al. EmbA is an essential arabinosyltransferase in Mycobacterium tuberculosis. Microbiology (Reading, Engl.) 2008
InteractionPhysicalInteraction Rv3794activepriti.priety2012-10-05

authors,LJ. Alderwick,HL. Birch,AK. Mishra,L. Eggeling,GS. Besra Structure, function and biosynthesis of the Mycobacterium tuberculosis cell wall: arabinogalactan and lipoarabinomannan assembly with a view to discovering new drug targets. Biochem. Soc. Trans. 2007
InteractionPhysicalInteraction Rv3794activepriti.priety2012-10-05

VE. Escuyer, MA. Lety et al. The role of the embA and embB gene products in the biosynthesis of the terminal hexaarabinofuranosyl motif of Mycobacterium smegmatis arabinogalactan. J. Biol. Chem. 2001
InteractionRegulatory Rv1267cactivepriyadarshinipriyanka20012012-10-05
Band Shift
K. Sharma, M. Gupta et al. Transcriptional control of the mycobacterial embCAB operon by PknH through a regulatory protein, EmbR, in vivo. J. Bacteriol. 2006
InteractionRegulatory Rv1266cactiveshahanup862012-10-05
Affinity purification (Physical interaction)
K. Sharma, M. Gupta et al. Transcriptional control of the mycobacterial embCAB operon by PknH through a regulatory protein, EmbR, in vivo. J. Bacteriol. 2006
InteractionRegulatedBy Rv1267cactiveyamir.moreno2012-10-05
qRT-PCR. mRNA expression levels of regulated element measured and compared between wild-type and trans-element mutation (knockout, over expression etc.) performed by using qRT-PCR technique. Electrophoretic mobility shift assays EMSA. Physical binding of the regulator to the regulated promoter proved by using electrophoretic mobility shift assay. .
K. Sharma, M. Gupta et al. Transcriptional control of the mycobacterial embCAB operon by PknH through a regulatory protein, EmbR, in vivo. J. Bacteriol. 2006
InteractionRegulatedBy Rv1267cactiveyamir.moreno2012-10-05
qRT-PCR. mRNA expression levels of regulated element measured and compared between wild-type and trans-element mutation (knockout, over expression etc.) performed by using qRT-PCR technique. Electrophoretic mobility shift assays EMSA. Physical binding of the regulator to the regulated promoter proved by using electrophoretic mobility shift assay. .
K. Sharma, M. Gupta et al. Transcriptional control of the mycobacterial embCAB operon by PknH through a regulatory protein, EmbR, in vivo. J. Bacteriol. 2006
InteractionRegulatedBy Rv1267cactiveyamir.moreno2012-10-05
Literature previously reported link (from Balazsi et al. 2008). Traceable author statement to experimental support.
G. Bal√°zsi, AP. Heath et al. The temporal response of the Mycobacterium tuberculosis gene regulatory network during growth arrest. Mol. Syst. Biol. 2008
NameDecaprenol phosphoarabinose-dependent arabinosyltransferase involved in the formation of the terminal hexaarabinofuranosyl motif of arabinogalactan; also proposed to elongate arabinan, probably as an alpha-1,5 arabinosyl transferase (although that is not fully established) that may form a multimer with EmbA; inhibition by ethambutolactivemjackson2012-10-05
Arabinosyltransferases
NameDecaprenol phosphoarabinose-dependent arabinosyltransferase involved in the formation of the terminal hexaarabinofuranosyl motif of arabinogalactan; also proposed to elongate arabinan, probably as an alpha-1,5 arabinosyl transferase (although that is not fully established) that may form a multimer with EmbA; inhibition by ethambutolactivemjackson2012-10-05
Arabinosyltransferases
TermEC:2.4.2.34 Indolylacetylinositol arabinosyltransferase. - NRactiveextern:JZUCKER2012-03-06
Inferred from direct assay
S. Berg, J. Starbuck et al. Roles of conserved proline and glycosyltransferase motifs of EmbC in biosynthesis of lipoarabinomannan. J. Biol. Chem. 2005
TermGO:0052636 arabinosyltransferase activity - NRactivemmcneil2012-03-05
Decaprenol phosphoarabinose-dependent arabinosyltransferase that elongates arabinan probably an alpha-1,5 arabinosyl transferase (although that is not fully established) that may form a multimer with EmbA inhibition by ethambutol
authors,JJ. Heymann,KD. Weaver,TA. Mietzner,AL. Crumbliss Sulfate as a synergistic anion facilitating iron binding by the bacterial transferrin FbpA: the origins and effects of anion promiscuity. J. Am. Chem. Soc. 2007
TermGO:0052636 arabinosyltransferase activity - NRactivemmcneil2012-03-05
Decaprenol phosphoarabinose-dependent arabinosyltransferase that elongates arabinan probably an alpha-1,5 arabinosyl transferase (although that is not fully established) that may form a multimer with EmbA inhibition by ethambutol
AE. Belanger, GS. Besra et al. The embAB genes of Mycobacterium avium encode an arabinosyl transferase involved in cell wall arabinan biosynthesis that is the target for the antimycobacterial drug ethambutol. Proc. Natl. Acad. Sci. U.S.A. 1996
TermEC:2.4.2.34 Indolylacetylinositol arabinosyltransferase. - NRactivejjmcfadden2012-03-05
Inferred from direct assay
S. Berg, J. Starbuck et al. Roles of conserved proline and glycosyltransferase motifs of EmbC in biosynthesis of lipoarabinomannan. J. Biol. Chem. 2005